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Hcpcs code for netspot gallium ga68 dotatate12/25/2023 ![]() In conclusion, we present a detailed, head-to-head comparison of the novel SST2-specific tracer DOTATATE and the validated metabolic tracer F-FDG for the evaluation of inflammation in small animal models of cardiovascular disease. In a translational PET/MRI study in atherosclerotic rabbits, we then compared Ga-DOTATATE and F-FDG for the assessment of aortic inflammation, combined with ex vivo radiometric assays and near-infrared imaging of macrophage burden. ![]() In both models, Cu-DOTATATE demonstrated preferential accumulation in macrophages with respect to other myeloid cells, while F-FDG was taken up by macrophages and other leukocytes. In atherosclerotic mice, Cu-DOTATATE PET aortic signal, but not F-FDG PET, was higher compared to controls (SUV max 1.1, IQR, 0.9–1.3 and 0.5, IQR, 0.5–0.6, respectively, N = 4, p = 0.0286). ![]() In mice subjected to myocardial infarction, in vivo Cu-DOTATATE PET showed higher differential uptake between infarcted (SUV max 1.3, IQR, 1.2–1.4, N = 4) and remote myocardium (SUV max 0.7, IQR, 0.5–0.8, N = 4, p = 0.0286), and with respect to controls (SUV max 0.6, IQR, 0.5–0.7, N = 4, p = 0.0286), than F-FDG PET. To evaluate differences in the tracers’ cellular specificity, we performed ensuing ex vivo flow cytometry and gamma counting. Additionally, we performed in vivo PET/CT experiments in mice with atherosclerosis, mice subjected to myocardial infarction and control animals, using both Cu-DOTATATE and F-FDG. DOTATATE’s pharmacokinetics and timed biodistribution were determined in control and atherosclerotic mice and rabbits by ex vivo gamma counting of blood and organs. For translational PET/MRI rabbit studies, we employed the more widely clinically used Ga-labeled DOTATATE, which was approved by the FDA in 2016. For mouse experiments, we labeled DOTATATE with the long-lived isotope Cu to enable studying the tracer’s mode of action by complementing in vivo PET/CT experiments with thorough ex vivo immunological analyses. In the current study, we performed a detailed, head-to-head comparison of DOTATATE-based radiotracers and F-FDG in mouse and rabbit models of cardiovascular inflammation. While the former is a widely applied metabolic tracer, DOTATATE is a relatively new PET tracer targeting the somatostatin receptor 2 (SST2). Clinically, two approved PET tracers hold great potential for inflammation imaging in cardiovascular patients, namely FDG and DOTATATE. In recent years, cardiovascular immuno-imaging by positron emission tomography (PET) has undergone tremendous progress in preclinical settings.
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